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| Registros recuperados : 12 | |
| 1. |  | GONCALVES, I. N.; UZÊDA, R. S.; LACERDA, G. A.; MOREIRA, R. R. N.; ARAUJO, F. R.; OLIVEIRA, R. H. M. de; CORBELINE, L. G.; GODIM, L. F. P. Molecular frequency and isolation of cyst-forming coccidia from free ranging chickens in Bahia State, Brazil. Veterinary Parasitology, v. 190, n. 1-2, p. 74-79, nov, 2012.| Biblioteca(s): Embrapa Gado de Corte. |
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| 2. | | RAMOS, R.; RAMOS, C.; ARAUJO, F. R.; OLIVEIRA, R. H. M. de; SOUZA, I.; PIMENTEL, D.; GALINDO, M.; SANTAN, M.; ROSAS, E.; FAUSTINO, M.; ALVES, L. Molecular survey and genetic characterization of tick-borne pathogens in dogs in metropolitan Recife (north-eastern Brazil). Parasitology Research, v.107, n. 5, p.1115-1120, Oct. 2010.| Biblioteca(s): Embrapa Gado de Corte. |
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| 3. | | RAMOS, C. A. N.; ARAUJO, F. R.; SOUZA, I. I. F.; OLIVEIRA, R. H. M. de; ELISEI, C.; SOARES, C. O.; ROSINHA, G. M. S.; ALVES, L. C. Caracterização molecular e antigenica de fosfoproteína ribosomal (P0) de Babesia bovis. In: SIMPÓSIO EMBRAPA LABEX DE SANIDADE ANIMAL, 1., 2009, Campo Grande, MS. Anais... Campo Grande, MS: Embrapa Gado de Corte, 2009. 2 p. 1 CD-ROM.| Biblioteca(s): Embrapa Gado de Corte. |
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| 4. | | SIQUEIRA, F.; TORRES JUNIOR, R. A. de A.; REGITANO, L. C. de A.; ALENCAR, M. M. de; SILVA, L. O. C. da; SOARES, C. O.; EUCLIDES FILHO, K.; ARAUJO, F. R.; ROSINHA, G. M. S.; OLIVEIRA, R. H. M. de. Determinação das frequências alélicas e genotípicas do gene da tireoglobulina em bovinos de corte. In: CONGRESSO BRASILEIRO DE CIÊNCIA E TECNOLOGIA DE CARNES, 4., 2007, Campinas, SP. Mercado do Século XXI: qualidade, segurança alimentar, certificação e rastreabilidade - Anais... Campinas: ITAL: CTC, 2007. p. 295-297.| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 5. | | RAMOS, C. A. N.; ARAÚJO, F. R.; SOUZA, I. I. F.; OLIVEIRA, R. H. M. de; ELISEI, C.; SOARES, C. O.; SACCO, A. M. S.; ROSINHA, G. M. S.; ALVES, L. C. Molecular and antigenic characterisation of ribosomal phosphoprotein P0 from Babesia bovis. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 104, n. 7, p. 998-1002, nov. 2009.| Biblioteca(s): Embrapa Pecuária Sul. |
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| 6. | | RAMOS, C. A. N.; ARAÚJO, F. R.; SOUZA, I. I. F.; OLIVEIRA, R. H. M. de; ELISEI, C.; SOARES, C. O.; SACCO, A. M. S.; ROSINHA, G. M. S.; ALVES, L. C. Molecular and antigenic characterisation of ribosomal phosphoprotein P0 from Babesia bovis. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 104, n.7, p. 998-1002, nov. 2009.| Biblioteca(s): Embrapa Gado de Corte. |
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| 7. | | FERREIRA, A. B. R.; TANURE, J. P. M.; ALMEIDA, J. G. de; DIEHL, L. de O.; SANTOS, M. G. dos; VILELA, M. de M.; ANDREU, M. P.; OLIVEIRA, R. H. M. de. Manual de boas práticas e segurança em laboratórios da Embrapa Gado de Corte. Campo Grande, MT: Embrapa Gado de Corte, 2017. 64 p. (Embrapa Gado de Corte. Documentos, 234).| Biblioteca(s): Embrapa Gado de Corte. |
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| 8. | | SOUZA, I. I. F.; RAMOS, C. A. N.; ARAUJO, F. R.; GUEDES JUNIOR, D. S.; OLIVEIRA, R. H. M. de; ELISEI, C.; ROSINHA, G. M. S.; SOARES, C. O. Caracterização molecular de isolados brasileiros de babesia bovis. In: JORNADA CIENTÍFICA DA EMBRAPA GADO DE CORTE, 4., 2008, Campo Grande, MS. Anais [da]... Campo Grande, MS: Embrapa Gado de Corte, 2008. 1 CD-ROM. Editores técnicos Valdemir Antônio Laura, Paulo Henrique Nogueira Biscola. 1 p. 1 CD-ROM.| Biblioteca(s): Embrapa Gado de Corte. |
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| 9. | | ARAUJO, F. R.; RAMOS, C. A. do N.; LUÍZ, H. L.; PÉRES, I. A. H. F. S.; OLIVEIRA, R. H. M. de; SOUZA, I. I. F. de; RUSSI, L. dos S. Avaliação de um protocolo de extração de DNA genômico a partir de sangue total. Campo Grande, MS: Embrapa Gado de Corte, 2009. 5 p. (Embrapa Gado de Corte. Comunicado técnico, 120).| Biblioteca(s): Embrapa Gado de Corte. |
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| 10. | | RAMOS, C. A. N.; ARAUJO, F. R.; SOUZA, I. F.; GUEDES JUNIOR, DANIEL S.; OLIVEIRA, R. H. M. de; FARIAS, T. A.; OLIVEIRA, J. B.; ALVES, L. C.; FAUSINO, M. A. Comparação entre diversos antígenos para o diagnóstico de Anaplasma marginale por ELISA. Pesquisa Veterinária Brasileira, v. 30, n. 1, p. 37-41, jan. 2010.| Biblioteca(s): Embrapa Gado de Corte. |
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| 11. | | RAMOS, C. A. N.; ARAUJO, F. R.; SOUZA, I. I. F.; BACANELLI, G.; LUIZ, H. L.; RUSSI, L. S.; OLIVEIRA, R. H. M. de; SOARES, C. O.; ROSINHA, G. M. S.; ALVES, L. C. Real-time polymerase chain reaction based on msa2c gene for detection of Babesia bovis. Veterinary Parasitology, v.176, p.79-83, Feb. 2011. Issue 1.| Biblioteca(s): Embrapa Gado de Corte. |
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| 12. | | FARIAS, T. A.; ARAUJO, F. R.; OSÓRIO, A. L. A. R.; JORGE, K. S. G.; RAMOS, C. A. N.; SOUZA, I. I. F.; OLIVEIRA, R. H. M. de; AZAMBUJA, A.; ROSINHA, G. M. S.; SOARES, C. O.; ELISEI, C. ELISA based on recombinant MPB70 to detect antibodies to Mycobacterium bovis. In: SIMPÓSIO EMBRAPA LABEX DE SANIDADE ANIMAL, 1., 2009, Campo Grande, MS. Anais... Campo Grande, MS: Embrapa Gado de Corte, 2009. 1 p. 1 CD-ROM.| Biblioteca(s): Embrapa Gado de Corte. |
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| Registros recuperados : 12 | |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Corte. Para informações adicionais entre em contato com cnpgc.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Gado de Corte. |
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Data corrente: |
17/12/2009 |
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Data da última atualização: |
19/03/2010 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
B - 1 |
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Autoria: |
RAMOS, C. A. N.; ARAÚJO, F. R.; SOUZA, I. I. F.; OLIVEIRA, R. H. M. de; ELISEI, C.; SOARES, C. O.; SACCO, A. M. S.; ROSINHA, G. M. S.; ALVES, L. C. |
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Afiliação: |
CARLOS ALBERTO DO NASCIMENTO RAMOS, UFRPE; FLABIO RIBEIRO ARAUJO, CNPGC; INGRID IEDA FERNANDO DE SOUZA, BOLSISTA - CNPGC; RENATO HENRIQUE MARCAL DE OLIVEIRA, CNPGC; CARINA ELISEI, BOLSISTA - CNPGC; CLEBER OLIVEIRA SOARES, CNPGC; ANA MARIA SASTRE SACCO, CPPSUL; GRACIA MARIA SOARES ROSINHA, CNPGC; LEUCIO C. ALVES, UFRPE. |
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Título: |
Molecular and antigenic characterisation of ribosomal phosphoprotein P0 from Babesia bovis. |
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Ano de publicação: |
2009 |
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Fonte/Imprenta: |
Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 104, n.7, p. 998-1002, nov. 2009. |
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Idioma: |
Inglês |
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Conteúdo: |
Babesia bovis is a tick-borne pathogen that remains an important constraint for the development of cattle industries in tropical and subtropical regions of the world. Effective control can be achieved by vaccination with live attenuated phenotypes of the parasite. However, these phenotypes have a number of drawbacks, which justifies the search for new, more efficient immunogens based mainly on recombinant protein technology. In the present paper, ribosomal phosphoprotein P0 from a Brazilian isolate of B. bovis was produced and evaluated with regard to conservation and antigenicity. The protein sequence displayed high conservation between different Brazilian isolates of B. bovis and several Apicomplexa parasites such as Theileria, Neospora and Toxoplasma. IgG from cattle experimentally and naturally infected with B. bovis as well as IgG1 and IgG2 from naturally infected cattle reacted with the recombinant protein. IgG from cattle experimentally infected with Babesia bigemina cross-reacted with B. bovis recombinant P0. These characteristics suggest that P0 is a potential antigen for recombinant vaccine preparations against bovine babesiosis. The aims of the present paper were to analyse the genetic conservation of the P0 gene among Brazilian isolates of B. bovis and produce recombinant P0 (rPO) from a Brazilian isolate and evaluate its antigenicity with sera from cattle naturally or experimentally infected with B. bovis and sera from cattle experimentally infected with B. bigemina in an enzyme-linked immunosorbent assay (ELISA). MenosBabesia bovis is a tick-borne pathogen that remains an important constraint for the development of cattle industries in tropical and subtropical regions of the world. Effective control can be achieved by vaccination with live attenuated phenotypes of the parasite. However, these phenotypes have a number of drawbacks, which justifies the search for new, more efficient immunogens based mainly on recombinant protein technology. In the present paper, ribosomal phosphoprotein P0 from a Brazilian isolate of B. bovis was produced and evaluated with regard to conservation and antigenicity. The protein sequence displayed high conservation between different Brazilian isolates of B. bovis and several Apicomplexa parasites such as Theileria, Neospora and Toxoplasma. IgG from cattle experimentally and naturally infected with B. bovis as well as IgG1 and IgG2 from naturally infected cattle reacted with the recombinant protein. IgG from cattle experimentally infected with Babesia bigemina cross-reacted with B. bovis recombinant P0. These characteristics suggest that P0 is a potential antigen for recombinant vaccine preparations against bovine babesiosis. The aims of the present paper were to analyse the genetic conservation of the P0 gene among Brazilian isolates of B. bovis and produce recombinant P0 (rPO) from a Brazilian isolate and evaluate its antigenicity with sera from cattle naturally or experimentally infected with B. bovis and sera from cattle experimentally infected with B. bige... Mostrar Tudo |
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Palavras-Chave: |
Babesiose bovina. |
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Thesagro: |
Babesia bovis; Bovino; Sanidade animal. |
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Categoria do assunto: |
H Saúde e Patologia |
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Marc: |
LEADER 02329naa a2200265 a 4500
001 1578251
005 2010-03-19
008 2009 bl --- 0-- u #d
100 1 $aRAMOS, C. A. N.
245 $aMolecular and antigenic characterisation of ribosomal phosphoprotein P0 from Babesia bovis.
260 $c2009
520 $aBabesia bovis is a tick-borne pathogen that remains an important constraint for the development of cattle industries in tropical and subtropical regions of the world. Effective control can be achieved by vaccination with live attenuated phenotypes of the parasite. However, these phenotypes have a number of drawbacks, which justifies the search for new, more efficient immunogens based mainly on recombinant protein technology. In the present paper, ribosomal phosphoprotein P0 from a Brazilian isolate of B. bovis was produced and evaluated with regard to conservation and antigenicity. The protein sequence displayed high conservation between different Brazilian isolates of B. bovis and several Apicomplexa parasites such as Theileria, Neospora and Toxoplasma. IgG from cattle experimentally and naturally infected with B. bovis as well as IgG1 and IgG2 from naturally infected cattle reacted with the recombinant protein. IgG from cattle experimentally infected with Babesia bigemina cross-reacted with B. bovis recombinant P0. These characteristics suggest that P0 is a potential antigen for recombinant vaccine preparations against bovine babesiosis. The aims of the present paper were to analyse the genetic conservation of the P0 gene among Brazilian isolates of B. bovis and produce recombinant P0 (rPO) from a Brazilian isolate and evaluate its antigenicity with sera from cattle naturally or experimentally infected with B. bovis and sera from cattle experimentally infected with B. bigemina in an enzyme-linked immunosorbent assay (ELISA).
650 $aBabesia bovis
650 $aBovino
650 $aSanidade animal
653 $aBabesiose bovina
700 1 $aARAÚJO, F. R.
700 1 $aSOUZA, I. I. F.
700 1 $aOLIVEIRA, R. H. M. de
700 1 $aELISEI, C.
700 1 $aSOARES, C. O.
700 1 $aSACCO, A. M. S.
700 1 $aROSINHA, G. M. S.
700 1 $aALVES, L. C.
773 $tMemórias do Instituto Oswaldo Cruz, Rio de Janeiro$gv. 104, n.7, p. 998-1002, nov. 2009.
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